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What is the relation of Beers Lambert's law in UV Visible spectrometer?
Wiki User
∙ 15y ago
The Beer-Lambert law
Absorbance = (extinction coefficent)(pathlength of light)(concentration)
allows you to measure the absorbance of sample in a UV spec, and change the rate from absorbance units / time to change in concentration / time.
the pathlength of light being the width of the cuvette
and the extinctin coefficent being specific to the product molecule.
Wiki User
∙ 15y ago
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