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in folin wu's method,in second step,we add molybic acid and sodium tungstate with sodium hydroxide and water in order to form phosphomolybdic acid.we heat the whole mixture so formed vigorously to remove ammonia present in the molybic acid.if it is not removed then it can form its complex with copper sulphate (alkaline) .due to this it will become unavailable to react with the given sample of deprotinised blood.and we cant proceed the glucose estimation further.
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