A group of people is presenting with a delayed onset of blisters and a dry productive cough that later turns productive. What is the likely culprit?
Radioimmunoassay was developed by Dr. Rosalyn S. Yalow and Dr. Solomon A. Berson in the early 1960s. This technique revolutionized the field of endocrinology by allowing the measurement of hormones in small quantities in biological samples.
Gel electrophoresis is used in forensic science to separate DNA fragments based on their size. It is a crucial technique for analyzing DNA evidence, such as identifying suspects or victims, matching samples, and determining genetic relationships. By running samples through a gel matrix under an electric field, researchers can visualize and compare DNA profiles for forensic investigations.
Medical laboratory technology and clinical laboratory science are similar in that they both involve testing and analyzing samples in a laboratory setting to aid in diagnosis and treatment of diseases. However, clinical laboratory science typically involves a broader scope of work, including more advanced testing techniques and the ability to interpret results. Medical laboratory technology may focus more on performing routine tests and procedures under the supervision of a clinical laboratory scientist.
In the external standard method for X-ray diffraction (XRD) technique, a known standard sample is used to calibrate the XRD instrument before analyzing unknown samples. The intensity of characteristic peaks from the standard sample is measured and used to calculate the correction factor or calibration curve, which is then applied to quantify the phases in the unknown samples based on their XRD patterns.
Brightfield microscopy is a common technique where light passes directly through a specimen, illuminating it and creating a bright background. It is often used for observing stained samples with high contrast. Brightfield microscopy is widely employed in biology to visualize cellular structures and study tissue samples.
The technique commonly used to test for T-2 mycotoxins in environmental and clinical samples is liquid chromatography coupled with mass spectrometry (LC-MS). This method allows for the sensitive and specific detection of T-2 toxins by separating them from other compounds and accurately quantifying their concentration. Additionally, immunoassays, such as enzyme-linked immunosorbent assays (ELISA), can also be employed for rapid screening of T-2 mycotoxins in various samples.
gas liquid chromatography-mass spectrometry technique
A common technique used to test for T-2 mycotoxins in environmental and clinical samples is liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). This method allows for the sensitive and specific detection of T-2 toxins by separating the compounds in a sample and quantitatively measuring them. Additionally, immunoassay methods, such as enzyme-linked immunosorbent assay (ELISA), can also be employed for rapid screening of T-2 mycotoxins in various samples.
A group of people is presenting with a delayed onset of blisters and a dry productive cough that later turns productive. What is the likely culprit?
T-2 mycotoxins can be tested using techniques such as liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) due to its high sensitivity and specificity. Additionally, enzyme-linked immunosorbent assay (ELISA) can be employed for screening purposes, providing a quicker and simpler method for detecting these toxins in environmental and clinical samples. Both methods allow for the effective quantification of T-2 mycotoxins in various matrices.
To test for T-2 mycotoxins in environmental samples, the technique of high-performance liquid chromatography (HPLC) coupled with mass spectrometry (MS) is commonly employed. This method allows for the separation, identification, and quantification of T-2 mycotoxins with high sensitivity and accuracy. Additionally, enzyme-linked immunosorbent assay (ELISA) can also be used as a rapid screening tool for detecting T-2 toxins in various matrices.
Laboratory diagnosis of stachybotryotoxicosis primarily involves the identification of Stachybotrys chartarum, the fungus associated with the condition, in environmental samples such as mold or dust. This can be achieved through culture methods, PCR assays, or microscopic examination. Additionally, biomarkers such as specific mycotoxins produced by the fungus may be detected in biological samples, including urine or blood, to support the diagnosis. Clinical evaluation of symptoms and exposure history are also essential components of the diagnostic process.
Assaying the samples in triplicate is another control. If you do not get the same result in all triplicate wells, you have a problem with your experimental technique or you have made a pipetting error. In a clinical laboratory, the experiment would have to be repeated.
From clinical samples (skin)
No. An extensive review of the scientific literature has revealed only one report of mycotoxins associated with two samples yeast products. This association was found in a product used in an animal feed manufacturing facility and could easily have been cross contaminated with another feed ingredient.
Clinical centrifuge are devices used for clinical applications like blood collection tubes. These laboratory equipment are driven by motor and spins liquid samples at high speed.
To determine if bacteria are present in environmental samples, you can perform tests such as culturing the samples on agar plates, using molecular techniques like PCR, or using biochemical assays to detect specific bacterial markers.