What would be the effect on product formation if the enzyme were heated to a temperature of 100 degrees celsius for 10 minutes before repeating the experiment?

Answer 1

They would be stopped because the enzyme is denatured from the high temperature. High temperature usually speed them up, but after 45 degrees celsius it becomes denatured.

Answer 2

Heating the enzyme to 100 degrees Celsius may denature it, causing the enzyme to lose its active conformation and hence lose its ability to catalyze the reaction effectively. This would likely result in a decrease in product formation or no product formation at all in the subsequent experiment.