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What cell walls are you using? In mammalian cell culture the reason you would use Ca/Mg free PBS is to help remove them from the surface they are attached to. The adherens proteins that adherent cells use to stick to whatever they are stuck to requires Ca2+ and Mg2+. Therefore if you simply want to wash the cells you have to use a buffer WITH Ca2+ and Mg2+.
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Can calcium displace from magnesium?
As calcium is more reactive than magnesium calcium will displace the magnesium and will become calcium nitrate and is left over with magnesium on it's own
What is the gas that reacts with calcium and magnesium when they burn?
Hydrogen gas is released when calcium and water react.
Would magnesium react with calcium nitrate?
no, it wouldn't calcium is above magnesium in the reactivity series which means that magnesium can't oxidize calcium. calcium can oxidize magnesium so calcium would react with magnesium nitrate with formation of metallic magnesium and calciumnitrate.
What is the difference between calcium hydroxide and Magnesium hydroxide?
the obvious difference is that one has calcium, and the other magnesium.
Where does calsium and magnesium come from?
Calcium comes from rocks in the earth crust so does magnesium but unlike calcium magnesium is obtained through salt water
Why do you wash cells with PBS before adding trypsin?
Because PBS removes Magnesium and Calcium ions which inhibit trypsin.
What is the differences between DPBS and PBS?
DPBS- Formulated by Dulbecco, Complete DPBS contians Calcium and magnesium. PBS- Which is used to wash the cells before trypsinization do not have calcium and magnesium and may have additional EDTA also. Typically there are many more combinations of Balanced salt solutions, So go by the formulations not by the name.
Is calcium with magnesium better for you?
A mixed calcium and magnesium supplement (such as dolomite) is better than taking just calcium or just magnesium.
Can calcium displace from magnesium?
As calcium is more reactive than magnesium calcium will displace the magnesium and will become calcium nitrate and is left over with magnesium on it's own
What is least reactive calcium magnesium radium strontium and barium?
calcium
What is the gas that reacts with calcium and magnesium when they burn?
Hydrogen gas is released when calcium and water react.
What 2 elements make water hard?
magnesium and calcium.
Would magnesium react with calcium nitrate?
no, it wouldn't calcium is above magnesium in the reactivity series which means that magnesium can't oxidize calcium. calcium can oxidize magnesium so calcium would react with magnesium nitrate with formation of metallic magnesium and calciumnitrate.
What is the difference between calcium hydroxide and Magnesium hydroxide?
the obvious difference is that one has calcium, and the other magnesium.
What is the measurement of the amount of calcium and magnesium which water contains?
The amount of calcium and magnesium in water determines its hardness.
Where does calsium and magnesium come from?
Calcium comes from rocks in the earth crust so does magnesium but unlike calcium magnesium is obtained through salt water
Would calcium nitrate and magnesium react?
Calcium nitrate and magnesium will not react since calcium is more reactive than magnesium and so, magnesium does not displace calcium (See Reactivity Series). However, if Calcium nitrate is in aqueous solution (dissolved in water), some bubbles may be seen when Magnesium is added to it, since the Magnesium would be reacting with the water and producing hydrogen bubbles.
