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What cell walls are you using? In mammalian cell culture the reason you would use Ca/Mg free PBS is to help remove them from the surface they are attached to. The adherens proteins that adherent cells use to stick to whatever they are stuck to requires Ca2+ and Mg2+. Therefore if you simply want to wash the cells you have to use a buffer WITH Ca2+ and Mg2+.
What else can I help you with?
Can calcium displace from magnesium?
Yes, calcium can displace magnesium in certain chemical reactions. This is more likely to happen when calcium is in a higher oxidation state than magnesium, allowing it to take the place of magnesium in a compound or reaction.
What is the gas that reacts with calcium and magnesium when they burn?
Hydrogen gas is released when calcium and water react.
Would magnesium react with calcium nitrate?
no, it wouldn't calcium is above magnesium in the reactivity series which means that magnesium can't oxidize calcium. calcium can oxidize magnesium so calcium would react with magnesium nitrate with formation of metallic magnesium and calciumnitrate.
Would calcium nitrate and magnesium react?
Calcium nitrate and magnesium will not react since calcium is more reactive than magnesium and so, magnesium does not displace calcium (See Reactivity Series). However, if Calcium nitrate is in aqueous solution (dissolved in water), some bubbles may be seen when Magnesium is added to it, since the Magnesium would be reacting with the water and producing hydrogen bubbles.
What two elements are close to magnesium in the periodic table of elements?
Beryllium, sodium, calcium are its neighbours.
Is calcium with magnesium better for you?
A mixed calcium and magnesium supplement (such as dolomite) is better than taking just calcium or just magnesium.
What is the differences between DPBS and PBS?
DPBS- Formulated by Dulbecco, Complete DPBS contians Calcium and magnesium. PBS- Which is used to wash the cells before trypsinization do not have calcium and magnesium and may have additional EDTA also. Typically there are many more combinations of Balanced salt solutions, So go by the formulations not by the name.
Can calcium displace from magnesium?
Yes, calcium can displace magnesium in certain chemical reactions. This is more likely to happen when calcium is in a higher oxidation state than magnesium, allowing it to take the place of magnesium in a compound or reaction.
What is the gas that reacts with calcium and magnesium when they burn?
Hydrogen gas is released when calcium and water react.
Would magnesium react with calcium nitrate?
no, it wouldn't calcium is above magnesium in the reactivity series which means that magnesium can't oxidize calcium. calcium can oxidize magnesium so calcium would react with magnesium nitrate with formation of metallic magnesium and calciumnitrate.
What 2 elements make water hard?
magnesium and calcium.
Would calcium be more chemically similar to potassium or to magnesium?
Calcium is more chemically similar to magnesium than to potassium. Both calcium and magnesium are alkaline earth metals, while potassium is an alkali metal. This means that calcium and magnesium have similar chemical properties due to their position on the periodic table.
What is the measurement of the amount of calcium and magnesium which water contains?
The amount of calcium and magnesium in water determines its hardness.
Why do you wash cells with PBS before adding trypsin?
Washing cells with PBS helps to remove excess media, serum, and debris before adding trypsin. This helps to increase the efficiency of trypsin digestion and ensures that the trypsin can effectively detach the cells from the culture vessel. Additionally, washing the cells with PBS helps to maintain cell viability during the trypsinization process.
What are the benefits of a magnesium-calcium formula?
High levels of calcium supplementation suppress magnesium absorption, so to overcome the magnesium deficiency that can be caused by high level calcium supplementation. Magnesium deficiency causes a decrease in the production, by the kidneys, of dihydroxy vitamin D-3 which results in inefficient absorption of calcium by the intestines and further loss of magnesium by the kidneys. Excess calcium in the body will result in calcium deposits such as kidney stones, arthritis...they will disappear after taking extra magnesium.
Do different types of magnesium react with calcium differently?
When you mean different type of magnesium, it can either be isotopes of magnesium or magnesium compounds. If you mean isotopes, i doubt any isotopes of magnesium is able to react with its calcium brethren. If you're talking bout magnesium compounds, reactivity between the metals depends on the electrochemical series, since magnesium is more electronegative, therefore i dont think calcium is able to displace magnesium from its compound. but magnesium is able to displace calcium from its compound.
What is the product of calcium and magnesium?
Clacium + Magnesium=> Carbon Dioxide + Water
