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Phenylalanine gives a yellow color with concentrated nitric acid due to the formation of a nitro derivative. Nitric acid reacts with the aromatic ring of phenylalanine, leading to the formation of a yellow compound.

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Why does the amino acid phenylalanine give a yellow color with concentrated nitric acid?

Phenylalanine gives a yellow color with concentrated nitric acid due to the formation of nitrophenylalanine derivatives. The concentrated nitric acid nitrates the phenylalanine, leading to the production of nitro groups that impart a yellow hue to the solution. This reaction is part of a broader class of chemical reactions involving amino acids and nitrous compounds, highlighting the interaction between amino acid side chains and strong acids.


What is the colour of concentrated nitric acid?

The nitric acid used in laboratory is colorless. The very concentated nitric acid - the fumans acid - with dissolved nitrogen oxides, can release white, red or brown fumes, very corrosive and toxic.


Why does concentrated HNO3 stain skin yellow?

Concentrated HNO3 (nitric acid) can react with the proteins in the skin to form a yellow-colored compound called xanthoproteic acid. This compound can absorb light in the visible spectrum, resulting in a yellow stain on the skin. This reaction is a chemical burn and should be treated immediately.


What tripeptide will give a positive xanthoproteic test?

Phenylalanine. When phenylalanine is subjected to a xanthoproteic test, it will produce a yellow solution due to the reaction of the aromatic ring with nitric acid, indicating the presence of phenyl group in the tripeptide.


How does albumin react with concentrated nitric acid?

Albumin reacts with concentrated nitric acid by undergoing nitration, where nitrogen atoms from the nitrate ions in the acid are added to the protein. This reaction can lead to denaturation of the albumin protein, resulting in loss of its biological activity and potential degradation of its structure.


Describe how to tell if a substance is a protein by using the xanthoproteic test?

Nitration of benzoid radicals present in the amino acid side chain occurs due to reaction with nitric acid giving the solutions a yellow colouration. This test is answered by aromatic amino acids like tyrosine and tryptophan. Phenyl alanine gives a weak positive reaction. When a preotein solution is heated with concentrated nitric acid, the benzene ring under goes nitration to form yellow nitro derivatives. When treated with NaOH the sodium salt formed is tense orange in color.


Why phenylalanine give negative result with xanthoproteic test?

Phenylalanine gave a yellow to orange color in xanthoproteic test which means it is positive.


Why is nitric acid yellow?

It is due to nitric dioxide gas contamination. Very pure nitric acid is clear because the synthesis of it removes it of nitric dioxide.


What would be the color of bromothymol blue in dilute nitric acid?

Bromothymol blue would appear yellow in dilute nitric acid.


What are the principles involved in xanthoproteic test?

The xanthoproteic test is a test for the detection of proteins. If proteins are present, concentrated nitric acid reacts with the proteins to form a yellow color that turns orange-yellow by the addition of alkali. This is called the xanthoproteic reaction.


Which substance turns yellow when nitric acid is dropped on it?

Potassium iodide turns yellow when nitric acid is dropped on it due to the formation of elemental iodine.


What is a mixture of concentrated nitric acid and concentrated hydrochloric acid is called?

This mixture is called Aqua regia (Latin: royal water) or aqua regis, which is a highly corrosive, fuming yellow or red solution, also called nitro-hydrochloric acid.The mixture is formed by freshly mixing concentrated nitric acid (12M) and concentrated hydrochloric acid (18M), usually in a molar ratio of 1:3 (volume ratio 1:2) respectively.Only this mixture is capable of dissolving gold (Au).Chemical reaction equation (Be aware of toxic fumes !):Au(s) + NO3-(aq) + 4 H+(aq) → Au3+(aq) + NO(g) + 2 H2O(l)