0
Why does the Anfinsen experiment not work using mature insulin?
Wiki User
∙ 10y ago
Proinsulin (a precursor to insulin) is made up of a polypeptide chain known in three parts as A, B and C. When proinsulin is converted to insulin the C part is cleaved off, leaving A and B attached only by disulfide bridges (C was in the middle of the polypeptide). In the Anfinsen experiment the beta-mercaptoethanol breaks these disulfide bridges, you now have two free, separate polypeptide chains. Thus there is no reason for them to go back together the way they originally were, just chance! It's not like a single polypeptide where the intermolecular interactions would determine how it refolded.
Wiki User
∙ 10y ago
Add your answer:
Earn +20 pts
A landmark experiment using the scientific method?
A Landmark experiment using the scientific method is... Research it for yourself!!!!
What is the solvent in an experiment?
The solvent is the liquid you will be using if you are dissolving something in the experiment.
What is the purpose of using malonic acid in this experiment?
it is a competitive inhibitor
What is the purpose of using anhydrous magnesum sulphate in the experiment?
removing water
What is the aim of the burning magnesium experiment?
when magnesium burn you find the number of moles by using the equation
How do you use an insulin pump correctly?
"When using an insulin pump, the reservoir needs to be filled with insulin. You do this by drawing insulin from a vial into the reservoir with the plunger, and then removing the needle."
Causation can be proved by using an?
experiment.
A sentence using the word experiment?
i hate experiment
A sentence using the word mature?
I am anxious to try these apples when they mature.
Can insulin be made by using transgenic bacteria?
Yes. In fact, currently insulin manufacturers worldwide use transgenic (recombinant) bacteria to produce insulin efficiently.
Diabetes Medicine?
Type I Diabetes is treated using insulin therapy. Insulin can be injected using many different methods, including syringes and insulin pumps. Type II diabetes medicine usually comes in pill form, and is combined with diet and exercise for better control.
A landmark experiment using the scientific method?
A Landmark experiment using the scientific method is... Research it for yourself!!!!
Make a sentence using hypothesis controlled experiment and variable?
Make a sentence using hypothesis, controlled experiment and variable. Make a sentence using hypothesis, controlled experiment and variable.
What is the solvent in an experiment?
The solvent is the liquid you will be using if you are dissolving something in the experiment.
Why must hormones like insulin and oxytocin be injected?
You can't take insulin by mouth because the acids in your stomach would destroy it. http://www.healthline.com/sw/khs-using-injected-insulin
How can the action of extracted insulin be prolonged?
Its action can be prolonged by using crystalline zinc insulin complexes, Lente preparations or protamine zinc insulin complexes. All of these complexes are in suspended form for subcutaneous administration.
Can you get so many insulin injections that your Diabetes is cured?
No. Diabetes is not cured by using insulin injections. You are giving yourself insulin injections because your body cannot produce enough insulin itself; therefore, if you were not to take the insulin injections you would have further detrimental complications. Once your body stops making insulin, it never "starts" making it again.
