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if it wouldn't be above the level of solvent the spots will run off

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15y ago
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13y ago

Having solvent levels below the origin give a more accurate spread of colours, as the water is allowed to begin properly soaking and ink is not lost in the solution upon direct contact. Also, there is little to no motion through the paper below the solvent level and hence, the ink/dye won't be able to travel up the chromatography paper.

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13y ago

because it will just end up dissolving in to the solvent and run of the paper before it even gets a chance to soak it's way up the paper.

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10y ago

because you won't be able to calculate the RF since you don't know what's the distance from the origin to the solvent front.

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11y ago

if the solvent front would be above the spot of dyes, than the dyes would washed out in solvent and would not be able to move.

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Q: Why is it important to stop the chromatograph before the solvent reaches the top of the paper?
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Why do we crush solids before putting them into a solvent?

to increase the surface area of the solute


Why do you crush solids before putting them into a solvent?

To increase the surface area of the solute, which allows it to dissolve faster.


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The solvent of jelly is water and the solute is jelly powder :) Hope it helped !


Why should the jar used to develop TLC plates be covered?

The jar should be covered so that it is saturated with the vapours of the volatile solvent before the plate is allowed to run.


What is the name of the solid residue remaining after evaporation?

Any residue present after evaporation of a solvent must have been either dissolved in the solvent, [by definition, permanently] suspended in the solvent, or a precipitate lying on the bottom of the solvent container, assuming that the solvent had not been disturbed for at least two hours. Regulatory samples, such as environmental samples taken from a lake or river for analysis, are typically shaken or stirred immediately before being passed through a filter with a 100 to 200 micron (micrometer) pore size to catch any precipitated solids. Weighing the filter before and after it has been used and allowing it to thoroughly dry will yield the mass of any precipitated solids. The first filtering is not always performed, and if it is, a filter with a larger pore size is sometimes used. These things depend on the specific procedure being followed and may also depend on whether there are any visible precipitated solids after the sample has been left undisturbed for several hours. Assuming that no precipitated solids are present or that the sample is either centrifuged or allowed to settle before some of it is carefully decanted off for further analysis, the only way to distinguish the mass of any suspended solids vs. the mass of any dissolved solids is to pass the sample through a filter with a 0.45 micron, or smaller, pore size. A filter with that small of a pore size will capture virtually anything suspended in a solvent. The mass of any suspended solids present may be determined by comparing the dried filter's mass before and after filtration. Comparing the mass of a sample dish containing an aliquot of the filtrate (the solvent that was filtered) before and after the solvent was allowed to completely evaporate allows the analyst to determine the percentage of dissolved solids.

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