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Ninhydrin is used in amino acid TLC because it readily stains amino acids by reacting with the amine groups. This reaction takes place very quickly and creates a brownish-color that can be easily visualized.
Wiki User
∙ 9y ago
Wiki User
∙ 12y agoNinhydrin (2,2-Dihydroxyindane-1,3-dione) is a chemical used to detect ammonia or primary and secondary amines, including amino acids. When reacting with these free amines, a deep blue or purple color known as Ruhemann's purple is produced.
Ninhydrin is not actually used in the separation process itself (is not part of the actual chromatography column), it is actually used to detect the separated compounds (e.g. amino acids). In clinical laboratories, for example, ion-exchange liquid chromatography systems are coupled with ninhydrin postcolumn derivatization systems before UV detection. Serum or urine is injected in the LC columns. After the amino acids are separates and eluted, they are passing through a ninhydrin derivatization system where they react (except proline) with ninhydrin leading to a purple solution that can be detected and quantified specifically at 570 nm.
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Why is TLC a poor technique for the analysis of low boiling liquids?
TLC is a slow technique so during the process the low boiling liquids become evaporated an we can't observe their presence.
Why KMnO4 used in TLC?
because it can make the point shown in your plate
What problem will ensue if the level of developing liquid is higher than the applied spot in a TLC analysis?
If the spots are submerged in the solvent, they are washed off the plate and lost.
What is difference between HPLC and TLC?
TLC= In it finely solid is spread on a rigid supporting plate (stationary phase)and the mobile phase is allowed to migrate across the surface of plate by capillary action. Less efficient then HPLCHPLC= in it column are used on place of plates and the mode of separation is adsorption or partition coefficient
Why do some compounds travel further on TLC plates than others?
it doesn't depends upon the TLC plates.. as it depends upon the solvents in which these compounds migrate.
Why you will spray ninhydrin for analysis in chromatography?
Since amino acids are colourless compounds, ninhydrin is used for detecting them. To identify this, after development, the TLC plate is sprayed with ninhydrin reagent and dried in an oven, at 105°C for about 5 minutes. Ninhydrin reacts with α- amino acids that results in purple coloured spots [(due to the formation of the complex - Rheuman's purple).
Why there appear to be differences in retention factor values under saturated and unsaturated conditions during TLC analysis?
tlc
Why is that TLC plates do not have stationary phases?
TLC is a method for qualitative analysis, which can be easily analyzed in normal sizes stationary phases.
Why is that TLC plates do not have long stationary phases?
TLC is a method for qualitative analysis, which can be easily analyzed in normal sizes stationary phases.
Why is TLC a poor technique for the analysis of low boiling liquids?
TLC is a slow technique so during the process the low boiling liquids become evaporated an we can't observe their presence.
What are the Spray reagents for visualize colerless compound in TLC?
Iodine vapours can be used as spray reagent in TLC
What is dimethylaminobenzaldihyde used for?
it is used for detecting Urobilinogen in Urine, in TLC plates, and for hydrazine indicator.
Why KMnO4 used in TLC?
because it can make the point shown in your plate
What problem will ensue if the level of developing liquid is higher than the applied spot in a TLC analysis?
If the spots are submerged in the solvent, they are washed off the plate and lost.
Was Beyonce ever in TLC?
No, Beyonce was never apart of the group TLC. She was in Destiny's child but never TLC
What channel is TLC on for regular cable?
44 TLC
How will one identify colourless compound on TLC?
Several possible ways:- place in an iodine chamber and many compounds will show up- look at plate in dark under uv light. Some compounds will show up because they absorb uv.- spray the plate with a chromogenic agent (like ninhydrin) to make the colorless compound colored.
