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Hey.
okay.. the biuret test is a test for peptide bonds. when CuSO4 is added to KOH, an alkaline solution, nitrogen atoms from the peptide bond forms a purple compound with the Cu2+ ions. hope this helps! read it in a text book.
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∙ 14y ago
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Does phenol give a positive biuret test?
No. The Biuret test only gives a positive result if a peptide bond exists. Phenol doesn't have a peptide bond, so it will not give a positive test.
Would glucose give a positive result with the Biuret test?
No because glucose does not consist of two or more peptide bonds
If Biuret and Millon's test give positive result what is the conclusion?
The conclusion is that the sample contain proteins.
What chemical reagent is used for proteins?
The biuret test tests for peptide (protein) bonds. In a test with a protein containing substance, a Copper(II) ion is reduced to Copper(I) which forms a compound with the nitrogen and carbon atoms of the peptide (protein) bonds in an alkaline (pH above 7) solution. If the solution goes to a purply-violet colour, proteins have been detected.
Will methionine give a positive Biuret test?
The answer is no. Not sure why at the moment but a question on my homework for biology lab asks why did lysine not react to the biuret's solution. If I had to guess it would be because Biuret's test is for peptide bonds in proteins and, lysine is just an amino acid not an actual protein, hence no peptide bonds. This is what I have concluded from just thinking about it. Not one hundred percent sure.
Does phenol give a positive biuret test?
No. The Biuret test only gives a positive result if a peptide bond exists. Phenol doesn't have a peptide bond, so it will not give a positive test.
Will glutamic acid give a positive biuret test?
Amino acids doesn't react with the biuret reagent.
Would glucose give a positive result with the Biuret test?
No because glucose does not consist of two or more peptide bonds
How many of these chemical groupings must be present in a molecule to give a positive biuret test?
you should have at least 2 of these,to obtain a positive result in Biuret's test. because biuret test is a test used to detect if there is peptide linkages.
If Biuret and Millon's test give positive result what is the conclusion?
The conclusion is that the sample contain proteins.
Will gelatin give a positive Biuret test?
yes Gelatin will have a positive biuret test. this is because it has an amide group which bends over the copper ion giving the colour pink or violet off to the visual eye.
How many chemical grouping must be present in a molecule to give a positive Biuret Test?
You must have at least two, those that will form the peptide linkage (NH2 and COOH) since Biuret test is for detecting peptide linkages.
What type of food will give a negative Iodine test and a positive Biuret Solution test?
An iodine test is used to test for the presence of starch (or polysaccharides, specifically amylose or amylopectin). A Biuret solution test is used as an indicator for peptide bonds within proteins. Therefore, if you get a negative iodine test and a positive Biuret test, you would probably be testing a animal food source (beef, chicken, pork). Overall, any food that doesn't have starch present within it would be appropriate to use. Hope this helps!
What chemical reagent is used for proteins?
The biuret test tests for peptide (protein) bonds. In a test with a protein containing substance, a Copper(II) ion is reduced to Copper(I) which forms a compound with the nitrogen and carbon atoms of the peptide (protein) bonds in an alkaline (pH above 7) solution. If the solution goes to a purply-violet colour, proteins have been detected.
Will methionine give a positive Biuret test?
The answer is no. Not sure why at the moment but a question on my homework for biology lab asks why did lysine not react to the biuret's solution. If I had to guess it would be because Biuret's test is for peptide bonds in proteins and, lysine is just an amino acid not an actual protein, hence no peptide bonds. This is what I have concluded from just thinking about it. Not one hundred percent sure.
Detect peptide bond?
by conducting the biuret test. the positive result of biuret test will give you the purple blue ring on top of the solution.(albumen/milk + cuso4 +NaOH)
You are given two test tubes and told that one contains a protein and the other amino acid. Explain fully how you would identify the test tube that contains amino acid?
This may be wrong but I think you could use the biuret test as this would only give a positive result in the test tube with protein in
