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Is the Bradford dye method of protein assay considered destructive or non destructive?
Wiki User
∙ 9y ago
non-destructive
Wiki User
∙ 9y ago
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What points should be considered when choosing labor saving devices?
warranty,convenience,care of method and voltage
What is the difference between the conventional method of dimensioning and the baseline method of dimensiong?
What is the difference between the conventional method of dimensioning and the baseline method of dimension
Which method is used to compute depletion?
Units of production method.
Technological advancement can come from improved devices or new?
Method.
Advantages and disadvantages using cyanmethemoglobin method?
The Cyanmethemoglobin method is a commonly used laboratory procedure for the estimation of hemoglobin in blood. This method has several advantages and disadvantages. Advantages: Accuracy: The Cyanmethemoglobin method is considered very reliable and is often used as a reference method. Stability: The Cyanmethemoglobin solution is stable, and readings can be taken at a later time without significant alteration. Interference: It is less susceptible to turbidity and lipemia interference as compared to other methods. Disadvantages: Toxicity: The reagent used, Drabkin's solution, contains potassium cyanide, which is toxic and needs to be handled with care. Time: The reaction time to obtain cyanmethemoglobin is relatively longer compared to other methods. Interference: The method can overestimate hemoglobin if a large number of white cells, such as in leukemias, are present. In conclusion, while the Cyanmethemoglobin method has its advantages, including accuracy and stability, it also has some disadvantages such as toxicity of the reagent used and potential interference in certain conditions. It's crucial to carefully consider these factors before deciding on a method for hemoglobin estimation.
What is the Bradford method?
The Bradford method involves a chemical test. It is used by scientists to determine the concentration of proteins in a solution.
What is the least destructive mining method?
The least destructive method would be to simply identify the desired mineral specimen by eye and pick it up.
Is lowry's protein estimation an invasive method?
No. It is a non-invasive method
Which method is best for determining Protein Quality?
Lowry method
How the protein sample without aromatic ring containing aminoacids can be observed under spectrophotometer and at what wave length?
A general protein assay for determining protein concentrations (of proteins with no aromatic amino acids) would be using the Bradford Method. The binding of this Coomassie Brilliant Blue G-250 dye to proteins will cause a shift in the dye's maximum absorbance wavelength. The free dye is red and absorbs at 465nm, but the blue protein-bound dye can be visualized at 595nm. You will need to construct a standard curve of BSA, for example. Then use the trend line from that curve to determine your protein concentration. Reference: Bradford, M. 1976, Analytical Biochemistry, vol. 72, p. 248.
How do you estimate proximate composition of fish?
proximate composition of fish include moisture, protein, fat, ash.moisture is estimated by oven method, protein by kjeldahl method, fat by soxhlet method ash by calcination method in the muffle furnace.
What has the author James M Bradford written?
James M. Bradford has written: 'A method for characterizing the surface cleanliness during adhesion testing' -- subject(s): Adhesion, Metal cleaning, Nickel
What is an invasive method of protein estimation?
gand mara
What is the standard reference method for protein determination?
Egg
What is an Ionic column?
it is a method used to purify protein
What is film radiography in non destructive testing?
It is a method to x-ray the object being tested.
Using benedict's reagent what method is used to find protein?
If the color is blue, it means that protein is present, if it turns orange, protein is not present.
