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In the layers pallet (usually this will be at the right side of the work space) select the layer you want to lock. Above the layers list you will see the word Lock and several icons; you can lock transparent pixels, lock image pixels, lock position, or lock all.

If the layers pallet is not visible go to the top menu and select window and then layers.
(the hotkey for this is F7)

This is for Photoshop CS4, an earlier version may be different

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