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When the application layer passes large amounts of data to the transport layer. The transport layer multiplex different applications' communication by dividing the application data into segments and placing a header on each segment to indicate which communication it is associated. Also, because networks can provide multiple routes that can have different transmission times, data can arrive in the wrong order. However by numbering and sequencing the segments, the transport layer can ensure that these segments are reassembled into proper order. Without segmentation, only one application would be able to receive data.

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