answersLogoWhite

0

Phenyl HPLC Columns

Ultra-low Bleed Phenyl Phase with Enhanced Phenyl Selectivity

Phenyl-based reversed-phases were one of the first alternatives to C18 selectivity. Our Ascentis Phenyl has been improved to offer exceptional phase stability and enhanced phenyl retention. Ascentis Phenyl offers versatility by also operating in the HILIC mode.

Features

  • Low-bleed for MS or UV gradient applications due to the use of trifunctional bonding reagent
  • Outstanding phenyl selectivity due to high phase loading and short butyl spacer
  • 100% aqueous-compatible for highly-polar compounds

Structure of Ascentis Phenyl

PropertiesUSP Code:L11Bonded Phase Description:Phenyl ring with short

butyl spacerEndcapped:YesParticle composition:Type B silica gelParticle Purity:<5 ppm metalsParticle Shape:SphericalParticle Size:3, 5 and 10 µmPore Size:100 ÅSurface Area:450 m2/gCarbon Load:19.5 %pH Range (recommended):2 to 8Extended pH range*:1.5 to 10

Key Applications

Small, water soluble molecules and peptides, π-acceptors, nitroaromatics, polar compounds, dipoles, heterocyclics, HILIC mode

Ordering Information for Ascentis Phenyl HPLC columns

Use

Phenyl phases are π-basic (electron donating) and are similar in overall retention to alkyl and EPG phases for easy column screening. The alternate selectivity of phenyl phases is often explained by the π-π interactions available through the phenyl ring. Compounds that appear to exhibit differential selectivity on the phenyl phase include:

  • hydrophobic bases (TCAs, tetracyclines)
  • moderate bases (anesthetics and narcotic analgesics)
  • benzodiazepines
  • some acidic compounds such as ACE inhibitors and quinoline antibiotics
  • nucleosides (e.g. cytidine)
  • nitro, azide and sulfonyl compounds

Notes

  • Methanol can be a more selective mobile phase component than acetonitrile.
  • Activate HILIC mode by using highly-organic (>90%) mobile phases.
User Avatar

Wiki User

13y ago

What else can I help you with?

Related Questions

What is the use of phenyl?

what is the use of phenyl ?


What are the uses of phenyl?

The phenyl is used in the hair care formulations and cosmetics.


Is phenyl a functional group?

Yes, phenyl is a functional group.


What is phenyl magnesium bromide?

it has phenyl group to which Mgbr attached at first position


How to make phenyl?

"Phenyl" is only part of a name, you'll need to be more specific.


How do you make phenyl?

WE HAVE WHITE PHENYL SOLUTION FROM WHICH YOU CAN MAKE UPTO 50 LITERS OF WHITE PHENYLE. ONLY YOU HAVE TO ADD WATER TO THE SOLUTION SLOWLY.CONTACT US AT 9414033040, 9314813795 , aditya.rawat@gmail.comHINDAUN CITY, RAJASTHAN, INDIA


What is the name of the subtituent C6H5 in a benzene ring called?

The substituent C6H5 in a benzene ring is called a &quot;phenyl&quot; group.


What is the common name for 3-phenyl-butanoic acid?

Its common name is B- phenyl butyric acid (B=Beta)


What is the Prepration of phenyl benzoate from phenol?

To prepare phenyl benzoate from phenol, first convert phenol to phenyl benzoate by reacting it with benzoic acid and a catalyst such as concentrated sulfuric acid or concentrated hydrochloric acid at elevated temperatures. This esterification reaction will yield phenyl benzoate along with water as a byproduct. Purification techniques such as distillation can then be used to isolate the desired product.


What is the electronegativity of phenyl salicylate?

Phenyl salicylate has a covalent electronegativity. This means that the chemical bonds in the substance&Ecirc;share electrons among the different atoms.&Ecirc;


How do you make phenyl benzoate from phenyl?

WE HAVE WHITE PHENYL SOLUTION FROM WHICH YOU CAN MAKE UPTO 50 LITERS OF WHITE PHENYLE. ONLY YOU HAVE TO ADD WATER TO THE SOLUTION SLOWLY.CONTACT US AT 9414033040, 9314813795 , aditya.rawat@gmail.comHINDAUN CITY, RAJASTHAN, INDIA


What is the formula for Phenyl Salicylate?

The formula is C13H10O3