A slide that includes a specimen that has been stained and glued to it is typically referred to as a "permanent slide" or "mounted slide." These slides are prepared for long-term storage and observation, allowing for detailed examination of the specimen's structure and features under a microscope. The staining enhances contrast, making cellular components more visible, while the glue or mounting medium preserves the specimen.
A stained specimen slide typically requires more light than an unstained live specimen slide. This is because the staining process can reduce the transparency of the specimen, making it harder for light to pass through and creating a need for more illumination to visualize details.
Most slides have to be either wet or stained in order to be properly viewed under a microscope. This includes potatoes, where if you want to see the starch, you will have to wet the slide and add stain.
Coverslip is not placed on a microscope but on the stained specimen on slide . This protects objective lens of microscope from getting stain from a wet mount . It also protects permanent slide .
The purpose of fixing a slide that is to be stained is to preserve the cellular structures and maintain the integrity of the specimen. Fixation helps to immobilize proteins and other cellular components, preventing degradation and maintaining morphology during the staining process. Additionally, it enhances the contrast of the specimen, making it easier to visualize specific structures or components under a microscope. Overall, fixing is a crucial step for accurate observation and analysis in microscopy.
Slide.
You need less light with a wet mount and more light with a stianed one. Barcteria, cells, etc. are almost always transparent. If you increase light in an unstained mount it will bleach out whatever it is youa re looking at.
the stage clips are used to hold the slide in place on the stage.
The slide containing the specimen is placed on the stage of the microscope. The stage is a flat platform where the slide sits, and it typically has clips to hold the slide in place. From the stage, the light source below illuminates the specimen for viewing through the lenses of the microscope.
Coverslip
To hold the specimen or slide
The specimen should be placed on the top surface of the microscope slide. This allows the light to pass through the specimen from below and be magnified by the lenses in the microscope to form an image for observation.
You place the specimen on a glass slide, which is then positioned on the stage of the microscope. The stage typically has clips to hold the slide in place during observation.