lavender top . tubes with other additives. tubes without additives
The proper order of draw for venipuncture is crucial to avoid cross-contamination of additives between tubes. The recommended sequence is: 1) Blood culture tubes, 2) Citrate tubes (light blue), 3) Serum tubes (red or gold), 4) Heparin tubes (green), 5) EDTA tubes (lavender), and 6) Glycolytic inhibitor tubes (gray). This order helps ensure accurate test results by minimizing the risk of interference from tube additives.
The order of draw for blood collection tubes typically starts with tubes that do not contain additives, such as the red top tube for serum samples. This is followed by tubes with specific additives for various tests, such as anticoagulants like EDTA or citrate for plasma samples. The specific order may vary depending on the facility's protocol.
Plain red top tubes do not require mixing at the time of collection. These tubes contain no additives or anticoagulants and are used for serum collection.
The correct sequence when obtaining multiple samples of blood is to collect samples in the following order: blood cultures, non-additive tubes (for serum), tubes with additives (e.g., EDTA, heparin, citrate), and finally, blood samples for coagulation studies. This sequence ensures that samples are not contaminated by additives from other tubes and prevents cross-contamination.
Pink or red tubes are commonly used for type and crossmatch blood draws. These tubes contain the necessary additives to prevent blood clotting and preserve the sample for compatibility testing.
There are typically two red top tubes used for blood collection in clinical settings: one containing no additives (plain tube) and one containing a clot activator. The specific number may vary based on the brand or type of tubes being used.
The correct order of draw according to the Clinical and Laboratory Standards Institute (CLSI) is blood culture tubes first, followed by coagulation tubes, then serum tubes, and finally tubes containing additives such as heparin or EDTA. This sequence helps minimize the risk of sample contamination and ensures accurate test results.
In capillary blood collection, the microcollection tube that should be filled last is typically the tube containing an additive, such as a gel separator or anticoagulant. This helps to prevent contamination of the sample with other additives from previously filled tubes. Often, the tube for hematology tests, which does not contain additives, is filled first, followed by tubes for biochemistry or other analyses.
Gray tubes containing sodium fluoride and potassium oxalate are commonly used to prevent glycolysis by inhibiting the enzymes responsible for breaking down glucose in the blood sample. These additives help preserve glucose levels for accurate testing in the laboratory.
To collect a serum specimen, you can use a serum separator tube (SST), which typically contains a gel that facilitates the separation of serum from blood cells during centrifugation. Other options include plain red-top tubes, which do not contain any additives. Both types of tubes should be allowed to clot before centrifugation to ensure optimal serum yield.
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