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How do you make 1x tae buffer?
Usually you need to dilute from a 50x stock. On that case dilute: For 500mL: 10mL 50x TAE + 490mL H2O For 1L: 20mL 50x TAE + 980mL H2O
How can you prepare H2SO4 0.25N?
To prepare a 0.25N solution of H2SO4, you would need to dilute concentrated sulfuric acid (typically around 95-98% purity) with water. For example, to prepare 1L of 0.25N H2SO4, you would mix 20.6mL of concentrated H2SO4 with approximately 980mL of water. Always add acid to water slowly and carefully while stirring to avoid splashing and heat generation.
How do make a 5.25N sulfuric acid solution from concentrated sulfuric acid solution?
con.H2SO4 is 98%(v/v)ie 980ml/litre.or 980X1.84(specific gravity of H2So4)ie wt/litre is 1803.2Normality= wt per litre/ Eq.wtie 1803.2/49=36.8 NHence con H2So4 is 36.8 NTo prepare 5 N , It has be diluted 7.36 times with water68
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