How protease may be solublized in water to increase brix value of juice?

Answer 1

Proteases can be solubilized in water by first dissolving the enzyme powder in a small amount of a neutral pH buffer or distilled water, ensuring it is fully hydrated. Once dissolved, the protease solution can be added to the juice, where it helps break down proteins that might otherwise form haze or cloudiness, leading to improved clarity and a higher Brix value. This enzymatic action can enhance juice quality by improving flavor release and mouthfeel. Proper temperature and pH conditions should be maintained for optimal enzyme activity.