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If blood is not available, a medical examiner can use alternative biological samples such as urine, breath, or even vitreous humor (the gel-like substance in the eye) to estimate the body's alcohol content. Each of these methods has its limitations; for instance, urine can yield variable results based on the time of consumption, and breath analysis relies on calibrated devices. However, with appropriate testing and calibration, these alternatives can provide a reasonable estimate of alcohol levels in the body.

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