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How increase the resolution between two peaks in high pressure chromatography?
Wiki User
∙ 11y ago
Resolution indicates the actual degree of separation of two compounds.
- It can be increased by;- increasing column length ( diffusion will increase proportionally , opposing resolution) - altering selectivity of the column, (mobile and stationary phases). also gradient elution.
Wiki User
∙ 11y ago
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When groundwater that flows in an aquifer between two layers of impermeable rock is forced to the surface by an increase in pressure .what is formed?
artesian well
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A cyclone rotates faster and faster as pressure differences between areas of low and high pressure increase.
What causes variations in the air pressure from place to place?
wind
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In chromatographic terms, TLC has great advantages over the other chromatography modes, such as Liquid Chromatography (LC), Column Chromatography (CC), Gas Chromatography (GC) and High Pressure Liquid Chromatography (HPLC).TLC's advantages are: (1) the ability to perform multiple analyses simultaneously; (2) speed and ease for scouting separation conditions, such as optimum solvent mixtures.
What is the difference between flash chromatography and column chromatography?
Column chromatography, is a broad term for all column chromatography methods, but is also synonomous with Gravity fed methods. Flash chromotography refers specifically to a column in which the eluant (or mobile phase) is moved through the column under pressure (using a hand pump for small scale, or a pressurised gas for a larger scale), the name Flash is derived from how much faster it is to run a column under pressure than via gravity.
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With the increase in flow rate the velocity of the fluid increases. and with the increase in velocity the pressure decreases, because there will be pressure drop (Refer Bernoulli's Theorm). So with increase in Flow rate the pressure decreases.
What is the difference between vacuum chromatography and flash chromatography?
Flash chromatography uses pressure (under 10 psi) to pump solvent down a column at a rate faster than gravity would provide. Vacuum chromatography uses a vacuum at the bottom of the column to pull solvent through. Both can be performed with standard glass columns, but usually vacuum chromatography is done with a silica filled vacuum funnel instead as a rough purification technique.
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The great the density the higher the air pessure.
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Principle of partition chromatography?
Chromatography in which separation is based mainly on differences be- tween the solubility of the sample components in the stationary phase (gas chromatography), or on differences between the solubilities of the compo- nents in the mobile and stationary phases (liquid chromatography)
What's the difference between blower and fan?
A blower is a machine for moving volumes of a gas with moderate increase of pressure. A fan moves large amounts of gas with low increase in pressure.
What is the difference between partition chromatography and adsorption chromatography?
The partition chromatography involves separation between liquids while adsoption chromatography involves solid and liquid separations. Answer: Partition Chromatography is a liquid liquid extraction which involves two solvents while adsorption chromatography is a liquid solid extraction which involves a solid stationary phase & a liquid mobile phase.
Similarities between gel electrophoresis and paper chromatography?
nothing
What is difference between resolution and vertical resolution?
Resolution is picture resolution and vertical resolution is number of pixels on y axis.
