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Some major cities located within or near the rainforest biome include Manaus in Brazil, located at the heart of the Amazon Rainforest; Puerto Maldonado in Peru, situated near the Peruvian Amazon Rainforest; and Iquitos, also in Peru, which is the largest city in the world that cannot be reached by road, surrounded by the Amazon Rainforest. These cities play a significant role in providing access to the rainforest for research, tourism, and resource extraction activities.

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