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NaCl is a constituent of many media require for the growth of mcirobial culture it has a significance that it provide the osmotic balance i.e. it helps in osmoregulation and also in transport of material in and out of the microbial cell (active transprot) Although it has been observed that in prokaryotic microbial cultures( except fungi) it is not necessary to add sodium chloride in basic media like nutrient agar because of the already presence of these ions in beef extract, yeast extract or other natural contituent.

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