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They can actually elute in either order. It depends on your mobile phase. In a weakly basic solution, the phosphate ions are found more as HPO4 (2-) rather than PO4 (3-) and thus will elute before sulfate which is larger and has the same charge.
In a strongly basic mobile phase, the PO4 (3-) ion will dominate, and will elute after the sulfate ion
What else can I help you with?
Do alkenes elute before alkanes in gas chromatography?
Usually, but not always. Depends on the bond position and polarity of the column. Alkenes are less likely to elute first on polar columns.
What is the first substance to elute in column chromatography?
The first substance to elute in column chromatography is typically the one that interacts the least with the stationary phase and moves through the column the fastest.
What is the elution order of compounds in column chromatography?
In column chromatography, compounds elute in order of increasing polarity. This means that less polar compounds will elute first, followed by more polar compounds.
Which of the compounds elute fast in reverse phase chromatography?
Compounds that are non-polar elute faster in reverse phase chromatography as the stationary phase is non-polar and retains polar compounds longer. Polarity of the compound determines its retention time in reverse phase chromatography.
What is the order in which compounds elute in column chromatography, with a focus on which compound typically elutes first?
In column chromatography, compounds elute based on their affinity for the stationary phase. Typically, compounds with weaker interactions with the stationary phase elute first, followed by those with stronger interactions. The compound that typically elutes first is the one with the least affinity for the stationary phase.
What is the basis of molecular sieve chromatography?
The purification in molecular sieve chromatography is dependent on the size of the molecules. The small molecules will enter into pores of gel while large molecules will be excluded from the pores.
How can you determine retention time of a compound?
Retention time of a compound can be determined using chromatography techniques such as gas chromatography or high-performance liquid chromatography. It is the time taken for a compound to travel through the chromatography system and elute from the column. By comparing the retention time of the compound of interest to known standards, the identification of the compound can be made.
What is molecular exclusion chromatography?
Molecular exclusion chromatography is a type of size exclusion chromatography that separates molecules based on their size and shape. It works by passing a sample mixture through a porous stationary phase, where smaller molecules are able to enter the pores and take longer to elute, while larger molecules pass more easily through the column and elute faster. This technique is commonly used for separating proteins and nucleic acids.
Retention time in Hplc?
Retention time in High Performance Liquid Chromatography (HPLC) refers to the time it takes for a compound to travel through the chromatography column and elute from the detector. It is a key parameter for identifying and characterizing compounds in a sample. Retention time is influenced by factors such as the column type, mobile phase composition, and compound properties.
Which phase is the reverse phase?
The reverse phase is the stationary phase in chromatography where nonpolar molecules elute faster than polar molecules. This is opposite to normal phase chromatography, where polar molecules elute faster than nonpolar molecules.
How can the order of elution for the gases be determined experimentally if the sequence is unknown?
To determine the order of elution for gases experimentally when the sequence is unknown, you can use gas chromatography. By analyzing the retention times of the gases as they pass through the chromatography column, you can identify the order in which they elute based on their unique characteristics.
What type of chromatography would be used to separate two proteins?
Size exclusion chromatography would be ideal for separating two proteins based on their size. This technique separates proteins by allowing smaller proteins to enter the pores of the stationary phase while larger proteins elute first.
