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To calculate the molecular weight of a protein from blood, you typically use techniques like size-exclusion chromatography or SDS-PAGE to separate the proteins based on size. After separation, you can compare the migration distance of the protein of interest with standard proteins of known molecular weights. Additionally, you can use the protein's amino acid sequence, where the molecular weight is calculated by summing the average molecular weights of the individual amino acids and accounting for water molecules released during peptide bond formation. The final molecular weight can be expressed in Daltons (Da).

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3w ago

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