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The Preparatory Reaction, also known as the link reaction or pyruvate decarboxylation, occurs in the mitochondria after glycolysis. The primary inputs are pyruvate (produced from glycolysis), NAD⁺, and coenzyme A (CoA). The outputs of this reaction are acetyl-CoA, carbon dioxide (CO₂), and NADH. This reaction serves as a crucial link between glycolysis and the Krebs cycle, facilitating the conversion of glucose-derived pyruvate into a form that can enter the Krebs cycle.

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