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Cot analysis was first developed and utilized in the

mid 1960s by Roy Britten, Eric Davidson, and

associates.

It is based upon the principles of DNA renaturation

kinetics.

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What is cot curve?

Cot curve is concerned with the measurement of the degree of reannealing of DNA strands. It is a curve drawn with X-axis having DNA concentration unit multiplied by time. Since the initial concentration is considered represented as Co and when multiplied with time t, it becomes "Cot" and the graph is known as Cot curve. The graph is drawn against %reanealled versus Cot.


What is cot curve why it is use?

it is the product of sample's nucleotide concentration(moles of nucleotides per liter) and reassociation time in seconds. it is used to study DNA renaturation kinetics to separate out the REPETITIVE DNA SEQUENCES from single low copy sequences.Repetitive DNA sequences renature at lowe Cot values than single copy sequences.


What is the simplified expression of (1-cot(x))squaredcot(x)?

To simplify the expression ((1 - \cot(x))^2 \cot(x)), we start by expanding ((1 - \cot(x))^2) to get (1 - 2\cot(x) + \cot^2(x)). Then, we multiply this by (\cot(x)): [ (1 - 2\cot(x) + \cot^2(x)) \cot(x) = \cot(x) - 2\cot^2(x) + \cot^3(x). ] Thus, the simplified expression is (\cot(x) - 2\cot^2(x) + \cot^3(x)).


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