The Hebrew alphabet originally had 23 letters from "aleph to ghah". Ghah meaning "dark, twisted, rope".
The ghah represented the mixing of the fallen "sons of God" mating with the daughters of men creating the mighty men of old, the men of renown, which represents the dna double helix (two or twin suns).
It was later blended with the "ayin" to represent high spin state dna that opens the third eye.
Yahushua, "the single brazen serpent" set on a staff, represents a single strand of DNA with 24 chromosomes: 23 chromosomes for man and 1 chromosome for God. 23 letters 23 chromosomes.
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Tav (ת) which has the sound of T
The last letter of the Hebrew Alphabet is Tav, ת, and it is pronounced like a T. In ancient times, it was pronounced like Th at the end of a word, but that has disappeared from Modern Hebrew.
There are only 22 letters in the Hebrew alphabet.
There is a highly supported theory about a missing Hebrew letter ghayin, but this letter would not have been the last letter of the alphabet. While this letter definitely existed in ancient Semitic languages, particularly Egyptian, and some modern Semitic languages such as Arabic, it no longer exists in any version of Hebrew.
Instead it has been absorbed into the letter ע (ayin). While the evidence exists showing that this is in fact a separate letter, there is very little evidence for reconstructing its original pictograph. The Ugarit and Arabic languages wrote this letter the same as the ayin but with an additional line or dot. The closest candidate for this letter is the Egyptian uniliteral symbol ? (a twisted rope), which is found in some ancient Semitic inscriptions.
Note some interesting examples of the missing ghayin:
Gaza in Arabic = Ghaza (غزة)
Gaza in Hebrew = ʿaza or Aza (עזה)
Gomorrah in Arabic = Ghamura (عمورة)Gomorrah in Hebrew = ʿamorah (עֲמֹרָה)
In the Arabic language this letter is called the ghayn and is probably related to the Hebrew word ghah meaning "twisted."
Because the Greek language transliterates this letter with a gamma ("g" sound) we know that this letter originally had a type of "g" sound, possibly a "ng" as in the word "ring" or the gh sound of the Arabic letter غ
If the letter ghayin were in the Hebrew alphabet today, it's likely it would have been written near the letter ayin, making it either the 16th or 17th letter.
Yod (י), pronounced like an English y sound. This letter is also used in the vowel combinations for ee, ay, and I.