Microemulsions are analysed in SEM in cryogenic mode. In that mode it is difficult to get a resolution of order of 40-50 nm(which is the typical domain size of microemulsions). So TEM is a better option
SEM 7nm or less TEM 0.5nm
Transmission electron microscopes (TEM) transmit electrons through a thin sample to create a detailed image of its internal structure, while scanning electron microscopes (SEM) scan a focused beam of electrons across the surface of a sample to create a 3D image of its topography. TEM is used for detailed imaging of internal structures at a nanometer scale, while SEM is used for surface imaging and analysis.
Transmission electron microscopes (TEM) use a beam of electrons transmitted through a thin sample to create an image, while scanning electron microscopes (SEM) use a beam of electrons scanned across the surface of a sample to create an image. TEM provides higher resolution images of internal structures, while SEM provides detailed surface images.
The process used to produce TEM will cut cells and tissues in to ultra-thin slices so that they can be viewed under the microscope. However, the ones on SEM do not need to be cut as they can easily be visualized.
SEM, TEM, and AFM are modern imaging techniques.
A scanning electron microscope (SEM) uses a focused beam of electrons to create detailed surface images of a sample, while a transmission electron microscope (TEM) transmits electrons through a thin sample to create detailed internal images. SEM is used for surface analysis, while TEM is used for studying internal structures at a nanoscale level.
In SEM (Scanning Electron Microscopy) you look at either backscattered or secondary electrones whereas in TEM (Transmission Electron Microscopy) you look how much of your electron beam makes it through the sample onto your phosphor screen or film camera. Usually SEM is used for surface analysis and TEM for analyzing sections.
1.Scanning electron microscope (SEM) 2.Transmission electron microscope (TEM)
Scanning Electron Microscopes (SEM) use a focused beam of electrons to create high-resolution images of a sample's surface, while Transmission Electron Microscopes (TEM) pass electrons through a thin sample to create detailed images of its internal structure.
light microscope or SEM or TEM electron microscopy
TEM images only have a view of the inner structure and are black and white so scientists don't actually know the colour of cells.SEM images are essentially a magnifies view of the specimens outer structure and are also black and white.Though both images can be contrasted via computers to add colour to see images more clearly
A transmission electron microscope (TEM) directs a beam of electrons through a thin specimen, producing a transmission image. A scanning electron microscope (SEM) scans a focused beam of electrons across the surface of a specimen, producing a 3D-like surface image based on electron interactions.