65 million years ago
Extinction of a single species can occur at any time if said species in hunted excessively, but mass extinction only occurs every 27 to 33 million years.
In the Late Cretaceous (65 million years ago).
The mass extinction of dinosaurs occurred around 66 million years ago during the Cretaceous-Paleogene (K-Pg) boundary.
540 million years ago was the first mass extinction
The Cretaceous–Tertiary extinction event 65 million years ago was the last mass-extinction, and many believe that humans are causing a mass-extinction right now.
Mass extinction could occur due to climate change, an asteroid impact and even a volcanic eruption.
Notable extinctions during the last mass extinction included all non-avian dinosaurs, pterosaurs, plesiosaurs, and ammonites.
No evidence of the extinct lifeforms appears in the stratigraphic record immediately following the extinction event.
Many geologists consider what you call the "last ice age" to be the last glacial of the present Ice Age, with the ice retreat starting only about 10-12ka. There was no mass extinction. A good many species did migrate or disappear altogther as the ice retreated, but in no way was it a mass extinction.
The three mass extinctions referred to are the Permian-Triassic extinction about 252 million years ago, the Triassic-Jurassic extinction about 201 million years ago, and the Cretaceous-Paleogene extinction about 66 million years ago.
The effect of mass extinction is extinction, death of a mass
500 years ago