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it is a labours process

it is timeconsuming process

it required more amount of dna require 3microliter dna per sampel

it is costly

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What are some other techniques that build on the Southern Blotting technique?

Some techniques that build on Southern blotting include Northern blotting for RNA detection, Western blotting for protein detection, and Southwestern blotting for DNA-binding proteins detection. These techniques are adaptations of Southern blotting and are used to detect specific molecules in biological samples.


What is the meaning of blotting technique?

Blotting technique is a laboratory method used to transfer biomolecules, such as DNA, RNA, or proteins, from a gel matrix to a membrane for further analysis. There are different types of blotting techniques including Southern blotting for DNA, Northern blotting for RNA, and Western blotting for proteins.


Difference between southern and northern blotting?

Southern Blotting refers to the identification of detailed sequences of DNA in which the DNA fragments are separated by electrophoresisNorthern Blotting refers to the identification of detailed sequences of RNA in which the RNA fragments are separated by electrophoresis


Is the principle of northern southern and western blotting is same?

yes the principle of all the three is same .the basic difference lies during probing nd detection .in case of southern blotting DNA is used in nothern rna and in case of western blotting antibodies are ued


Which describes the transfer of mRNAs to a membrane to analyze gene expression?

southern blotting


What is blotting and types of blotting?

Blotting is a technique of transferring DNA or RNA or Protein from a gel to the membrane. nitrocellulose, PVDF or nylon membrane are used to attach these molecules permanently on them. Western, southern and norther blotting are the techniques used to transfer protein, DNA and RNA respectively. It is done to detect them with a specific probe or antibody. This can not be detected or easy to handle on the gel, so we do blot for these analysis.


Is etbr transfer with DNA during southern blotting?

The answer is NO! To me, it makes no sense, why we have to transfer etbr with DNA to a blotting membrane? When Southern blotting signals will be detected by means of Radiochemicals or fluro labelling why we have to think about EtBr?


What is a blad?

A blad is a Scots term for a portfolio, blotting-book or blotting-pad.


Can chlamydia cause blotting?

Blotting? As In Bleeding, then yeah It doesYou can bleed from being infected by chlamydia.


What were the advantages and disadvantages of building more towns in the southern colonies?

Colonists


What is were the advantages and disadvantages of building more towns in the southern colonies?

Colonists


What is blotting technique?

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