answersLogoWhite

0

1 Isolate DNA

2 Cut DNA with a restriction enzyme

3 Mix the DNA's and join then together by using DNA ligase

4 Insert the recombinant plasmid into a host bacterium

5 Allow the bacterium to reproduce

User Avatar

Wiki User

15y ago

What else can I help you with?

Related Questions

What Genetic engineering can be accomplished in bacteria using?

Genetic engineering involves the use of recombinant DNA technology, the process by which a DNA sequence is manipulated in vitro, thus creating recombinant DNA molecules that have new combinations of genetic material


What is the substance required to cleave the vector DNA during recombinant DNA technology?

Restriction enzymes are the substances required to cleave the vector DNA during recombinant DNA technology. These enzymes recognize specific DNA sequences and cut the DNA at specific points, allowing for the insertion of foreign DNA fragments.


What is a biotherapeutic?

A biotherapeutic is a therapeutic material produced using biological means, including recombinant DNA technology.


Describe one similarity between PCR and recombinant DNA technology.?

PCR is the abbreviation for polymerase chain reaction. It is similar to recombinant DNA technology in that both have the ability to sequence DNA.


Why cleavage of DNA is done in recombinant DNA technology?

Recombinant DNA technology is the most emerging technique for the production of DNA for the useful bio-materials like insulin. So to produce recombinant DNA two different DNA is rejoined. so cleavage is done to extract the desired DNA and then joined again.


Why was the discovery of restriction enzymes important to recombinant DNA technology?

Recombinant DNA technology requires fragments of DNA from the source genome. Using crude methods such as mechanical shearing, we get random fragments of DNA, and their sequence is unknown. Restriction enzymes are specific in site recognition and cutting and their discovery lead to proper fragments of DNA which have some known sequences.


What is a DNA molecule containing regions from different sources?

A DNA molecule containing regions from different sources is called recombinant DNA. This is often created in laboratories by combining DNA from different organisms or through genetic engineering techniques. Recombinant DNA technology has many applications in biotechnology and genetic research.


What genetic technology is primarily responsible for the genetic technology revolution?

recombinant DNA


How is PCR and recombinant DNA technology similar?

PCR and recombinant DNA technology both involve manipulating DNA in the laboratory. PCR is a technique used to amplify specific DNA sequences, while recombinant DNA technology involves combining DNA from different sources to create a new DNA molecule. Both techniques have revolutionized the field of molecular biology and have numerous applications in research and biotechnology.


What is the final product of recombinant DNA technology?

Typically a protein.


Methods of microbial strain improvement?

recombinant DNA technology


Definition of biotherapeutics?

The field encompassing therapeutic materials produced using biological means, including recombinant DNA technology.