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Diafiltration is a membrane filtration process used to separate and purify solutes from a solvent, typically in the context of bioprocessing and water treatment. It involves the addition of solvent to a concentrated solution while simultaneously removing permeate, effectively diluting the solute concentration while retaining it on one side of the membrane. This technique is often employed to remove small impurities or to concentrate desired biomolecules, enhancing the efficiency of purification processes.

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