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an assay that measures the appearance or disappearance of a fluorescent.

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12y ago

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Estimate Serratiopeptidase in Pharmaceutical products Is there a spectrophotometric method available?

spectro assay of serratiopeptidase


What amino acid residues are detected when the spectrophotometric assay is used to quantify proteins?

In the spectrophotometric assay for protein quantification, the method typically detects tyrosine, tryptophan, and cysteine residues due to their absorption at specific wavelengths. These amino acids have distinct absorption spectra that enable the measurement of protein concentration based on the amount of light absorbed.


Spectrophotometric methods using light?

For example UV-VIS absorption spectrophotometry.


What is the principle of hemoglobin estimation using cyanmethemoglobin method?

what are the various methods of the Hb estimation???/kindly tell me the senstivity of the methods. i know only one method sahli hemoglobinometer(acid hematin) method. I have enumerated few Sahli - Adam's Method Dare's method Haden's method Wintrobe's method Haldane's method Talliquist method Gasometric method Spectrophotometric method Automated & non-automated hemolglobinometry Other methods alkaline hematin method specific gravity comparator method


Is absorbance depends on the temp of the room or solvents in spectrophotometric measurement?

Absorbance is generally not significantly affected by small changes in temperature of the room or solvents. However, drastic changes in temperature can impact the optical properties of the solution, potentially affecting absorbance measurements. It is important to maintain consistent temperature conditions during spectrophotometric measurements for accurate results.


What would happen when sulphuric acid is not added in spectrophotometric titration?

If sulfuric acid is not added in spectrophotometric titration, the acidic conditions required for the reaction between the analyte and the titrant may not be met. This could lead to inaccurate results or incomplete reaction, affecting the precision and reliability of the titration. Additionally, the absence of sulfuric acid may alter the chemistry of the system and prevent the formation of the colored complex that is usually measured in spectrophotometric titrations.


What is spectrophotometric titration?

Spectrophotometric titration is a technique that combines the principles of spectrophotometry and titration to determine the concentration of a specific analyte in a solution. It involves measuring the absorbance of a sample as a titrant is added in incremental amounts, leading to a titration curve that can be used to calculate the concentration of the analyte.


Which are the Methods for hemoglobin estimation?

Hemoglobin estimation is the measuring of hemoglobin levels that are broken into three categories. Visual methods are Sahil, Dares, Hadens, Wintrobes, Haldanes, Tallquists method. Second to consider are the gasometric method, single to the Van Slyke method. Lastly the spectrophotometric method oxyhemoglo, and the cyanmethemoblobin method.


How can an icteric hemolyzed or lipemic sample be corrected for total protein assay?

An icteric sample can be corrected by using a method that does not rely on colorimetric detection, such as turbidimetric or refractometric methods. Hemolysis can be corrected by using a spectrophotometric method that measures protein concentration at a wavelength where hemoglobin absorption is minimal. Lipemia can be corrected by using ultracentrifugation or filtration to remove the lipids before analyzing the sample for total protein.


I want to analyze nitrate and nitrite concentration in food products How can I prepare Griess reagent for spectrophotometric method?

why would you ask a question like this to the Internet? if you want to know you should probably contact a chemist or something...you need help. my answer to your question is...no one knows and that's why it hasn't been answered yet.


Why is standard plate count an indirect measurement of cell density whereas spectrophotometric analysis is not a count at all?

go ask your mom


What is the formula on how to find starch in water?

To find the concentration of starch in water, you can use a spectrophotometric method by measuring the absorbance of the solution at a specific wavelength. Prepare a standard curve using known concentrations of starch solutions to correlate absorbance with concentration. Then, measure the absorbance of your sample and use the standard curve to determine the starch concentration.