This refers to the type of detergent used to lyse cell membranes when extracting DNA from cells. SDS=Sodium dodecyl sulfate, CTAB=Cetyl trimethylammonium bromide
Special Direct System drills are heavier than regular drills, have a hammer action and a chiselling action and take larger drill bits and chisels.
The SDS-plus is the most common shank for bits that fit medium sized hammer drills. It was developed by Hilti and the initials mean "insert, twist, secure" in German.
The SDS system is used on drills that drill through stuff like concrete. The bit has a standard size male part with four grooves in it. The drill chuck has four fingers that mate with the grooves on the bit shank. To change bits you just pull the old one out of the drill with your hand and push the new one into the chuck. It's very slick. They even sell a Jacobs-style jaw chuck adapter for it, so you can use all your non-SDS bits with an SDS drill.
Phenol chloroform extraction is the oldest and still widely followed method for the isolation and extraction of DNA from plant and animal cells. The phenol, chloroform (and also isoamyl alchohol) are added in a specific ratio of 25: 24:1.Phenol: Phenol dissolves the organic impurities, like proteins etc.chloroform: Provides density to phenol so that it settles below water during phase separation.Isoamylalchohol: Used to prevent phosgene from reaction of chloroform with air.The Phenol:Chloroform:Isoamylalchohol (PCI) solution is added to the cell extract after removal removal of debris. After proper mixing, cetrifugation is done to separate the phases. Two phases are formed: The upper, the aqueous phase that contains DNA, the lower phase, that phenol phase, that contains organic impurities. Thus two phases are separated by a very clearly defined boundary of coagulated proteins.The aqueous phase is precipitated and then the DNA could be pelleted after rounds of purifications.
It's a special tough drill that fits rotary hammer drills like Hilti and Bosch.
In CTAB method,SEVAG is used to breakdown the tissues in the extracted leaves.While in dellaporta method,the SDS and POTASSIUM ACETATE are used.In CTAB method BLUECAP/TEST TUBES are used,while in dellaporta method the EMPENDORFS are mostly used.ICE COLD ETHANOL is used mostly in the CTAB method for resuspension,while in dellaporta method ISOPROPANOL is used.
CTAB is a cationic detergent. It is generally considered to be less denaturing than SDS. It is typically used when it is desired to maintain enzymatic activity. It is also typically used in protein refolding.
SDS Plus drill bits have a stronger grip and are more suitable for heavy-duty drilling tasks compared to regular SDS drill bits. The key difference lies in the design of the shank, with SDS Plus having a more robust connection to the drill.
The SDS drill is considered to be the superior option for efficiency and ease of use, it does not require a chuck key. The hammer drill requires a chuck key for fitting different drill bits, which is considered the main difference of the drills.
SDS-PAGE method
The short answer to your question is "yes". I found myself researching the same question a few days ago and found that the real difference is between SDS/SDS Plus and SDS Max. I don't recall the exact dimension now, so I won't try to quote it, but the Max is a larger size. The answer I found was enough to tell me I used SDS (SDS Plus), and those were the bits I needed to buy. Once I knew that, I didn't need to remember the size of SDS Max...they were too big for my drill. Last point, SDS Plus is sometimes shortened to SDS+.
Electrophoresis is the method that could be used to further separate two bands from the same protein fraction after SDS-PAGE.
SDS drill bits have a cylindrical shank while SDS Plus drill bits have a grooved shank that allows for a stronger grip in the chuck. SDS Plus drill bits are also longer and have a higher drilling capacity compared to SDS drill bits.
The key differences between SDS Plus and SDS Max drill bits are their size and power. SDS Max drill bits are larger and more powerful than SDS Plus drill bits, making them more suitable for heavy-duty drilling applications.
An SDS drill bit is a type of drill bit that is designed for use with SDS drills, which are power tools commonly used for drilling into concrete and masonry. The main difference between SDS drill bits and other types of drill bits is that SDS drill bits have a special shank design that allows them to be quickly and securely inserted into the chuck of an SDS drill without the need for tightening or loosening a chuck key. This makes them more convenient and efficient for heavy-duty drilling tasks.
slab gel with and without SDS
SDS-PAGE is a technique used to separate proteins based on their size, while western blotting is a technique used to detect specific proteins in a sample using antibodies. In SDS-PAGE, proteins are separated by gel electrophoresis, while in western blotting, proteins are transferred from a gel to a membrane for detection using antibodies.