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Senescence is the period between maturity and death; as it relates to tissue culture this is the phase when confluence has been reached (assuming anchorage-dependant cells) and when the media contents contain waste and/or factors to an extent which causes the cells to begin to die-off en masse. The point of tissue culture, typically, is to avoid the senescence phase by regularly changing the media (to dispose of waste products which have toxicity effects), and by subculturing while in log-phase (before the chemical signalling that can cause senescence begins).

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What is difference between tissue culture cell culture and organ culture?

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The scientific definition of tissue culture originally meant the growth and maintenance of tissue pieces. Now it is more commonly referring to the technique of the cell culture.


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