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You certainly can use a helping verb, but it depends on the tense. If it's a simple past tense, you don't need one: Who ate my sandwich? Who took my pen? But if you are using a past perfect tense, the helping verb is necessary, to show an action that happened earlier in the past: Who has seen this movie? Who has read the chapter?

Other examples of interrogative sentences with who asking the question but no helping verb:

Who is Alice?

Who are you?

Who was the one?

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Q: Do we use helping verb with who in interrogative sentence?
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