Clinical and Laboratory Standards Institute
clsi
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CLSI
clsi
Clinical and Laboratory Standards Institute
The Clinical and Laboratory Standards Institute (CLSI) is responsible for providing guidelines on the recommended order of draw for blood collection. They publish standards and best practices to ensure accurate and reliable laboratory test results.
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The correct order of draw according to the Clinical and Laboratory Standards Institute (CLSI) is blood culture tubes first, followed by coagulation tubes, then serum tubes, and finally tubes containing additives such as heparin or EDTA. This sequence helps minimize the risk of sample contamination and ensures accurate test results.
The Clinical and Laboratory Standards Institute (CLSI) recommends a maximum depth of heel puncture not to exceed 2.0 mm. This guideline is aimed at minimizing the risk of injury to the underlying structures, such as bones and nerves, especially in neonates and infants. Proper technique and adherence to this depth are crucial for safe and effective blood sample collection from the heel.
According to the Clinical and Laboratory Standards Institute (CLSI), it is unacceptable to use alcohol alone to disinfect the site prior to blood culture collection. This is because alcohol does not effectively kill all pathogens, particularly some bacterial spores. CLSI recommends using a chlorhexidine-based antiseptic or iodine-based solution for proper skin disinfection before collecting blood cultures to ensure optimal contamination prevention.
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The Clinical and Laboratory Standards Institute (CLSI) and the American Society for Clinical Pathology (ASCP) recommend a specific order of draw to prevent cross-contamination of samples. The general order is as follows: blood culture bottles, light blue top (sodium citrate), red top (serum), gold or tiger top (serum separator), green top (heparin), lavender top (EDTA), and gray top (glycolytic inhibitor). Following this order ensures that additives in the collection tubes do not interfere with test results. Always refer to the latest guidelines for any updates or specific protocols.
The Clinical and Laboratory Standards Institute (CLSI) recommends the order of draw as: 1) Blood culture tubes, 2) Coagulation tubes, 3) Serum tubes, 4) Heparin tubes (plasma), 5) EDTA tubes. The Association of Phlebotomy Technicians (ASPT) follows a similar order with minor variations depending on the institution's protocol. It's important to follow the specific guidelines of the facility where you are working to ensure accuracy and prevent sample contamination.