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optimization means providing the perfect conditions for taq pol to bind and thus a smooth reactionb that amplifies our sample................this includes optimizing the amplification temp, the pH of buffer , the purity chech of the DNA that's to be amplified,adding correct amount of enzyme etc...............for additional information do refer to sambrook and rusell lab manual page 155

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Q: What is the meaning of optimization of PCR with respect to specific parameters?
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