You need to have some conflict as soon as possible, once you introduce your characters and the basic setting.
Expostition
The conflict is typically introduced in the exposition of a story. This is where the setting, characters, and background information are established, laying the foundation for the conflict that will drive the story forward.
A force that has been placed in proximity to a conflict or potential conflict and has not been introduced to hostilities.
A story begins with exposition, where you are introduced to the characters and setting and conflict.
character vs. nature
In "Amigo Brothers," the conflict is introduced as two friends, Paco and Antonio, must box each other in a competition. The conflict is developed through their internal struggle of potentially hurting each other while fighting. The story ends without a clear resolution, leaving the outcome of the fight to the reader's interpretation.
The part of a story we call "rising action" is that part where the tension and conflict increase until they reach the climax or peak of the story.
That part is typically called the inciting incident. It is the moment in the story where the main conflict or problem is introduced, setting the events of the plot in motion.
Conflict flows throughout the a plot. It starts in the exposition stage, where the author lays out the plot. During this stage the characters, settings and conflict are all introduced.
The exposition is where the main characters and setting are introduced.
an outline of characters and setting which prepares the reader for the conflict.
The event in the story that can be best described as the inciting incident is the moment when the main conflict or problem is introduced, setting the story in motion.