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You can adjust the fine focus knob to bring the onion into sharper focus. If the image is still not clear, you may need to also adjust the condenser or the diaphragm to optimize the lighting.
You should adjust the fine focus knob to sharpen the image when viewing the onion cell under high-power objective (HPO) on a microscope. Rotate the knob gently to focus the specimen until the image appears clear.
The hypothesis of observing onion cells under a microscope could be that the onion cells will have a distinct rectangular shape and contain a visible nucleus.
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When observing an onion cell under the microscope, it appear to be long an oval in shape. Staining the cell will make the chromosomes attain a deep purple color.
Ron is observing an onion cell on a slide under a microscope. He sees chromatids being pulled to opposite ends of the cell. Which phase is he seeing?
To prepare an onion cell slide for microscopic examination, first peel off a thin layer of the onion skin. Place the thin layer on a microscope slide and add a drop of water to the onion skin. Gently place a coverslip over the onion skin to create a flat surface for viewing under the microscope. Finally, carefully place the slide on the microscope stage and adjust the focus to observe the onion cells.
When observing an onion cell under the microscope, it appear to be long an oval in shape. Staining the cell will make the chromosomes attain a deep purple color.
You should use iodine to stain the cells. Set the cells on a slide, let them dry you will repeat the process until it is completely covered in iodine and then you will slide it in the stage and then hopefully you will then see the sells of the onion skins.
Onion epidermis is a suitable tissue for observing cellular structures with a compound light microscope because it is transparent, allowing light to pass through easily, and the cells are thin enough to view individual cells and structures such as cell walls, nuclei, and chloroplasts. Additionally, the large size of onion cells compared to other plant cells makes it easier to study their internal structures.
Under a compound light microscope, an onion cell will appear as a rectangular shape with a distinct cell wall and nucleus. The cell cytoplasm will be visible, with small structures such as vacuoles dispersed throughout. The onion cell may also have a clear membrane visible around the cell wall.
The key characteristics of an onion cell observed under a microscope include a rectangular shape, a cell wall that appears as a thin, clear outline, a large central vacuole that takes up most of the cell's space, and small, dark-stained organelles called nuclei.