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Babies are not born with a fully developed microbiome; instead, they begin acquiring their microbiota during birth and shortly after. During vaginal delivery, they are exposed to their mother's vaginal and intestinal bacteria, while cesarean-delivered babies may initially have a different microbial profile. Additionally, breastfeeding further influences the development of a baby's gut microbiome, as breast milk contains beneficial bacteria and prebiotics. Over time, the microbiota diversifies and matures as the baby is exposed to different environments and foods.

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