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No, Mary Queen of Scots (1542 - 1587), was not proven to have killed a family member of Elizabeth I (1533 - 1603). Although it was suspected she was complicit in the death of her second husband, Lord Henry Darnley (1545 - 1567), and as Darnley's grandmother was Margaret Tudor, Elizabeth's aunt, that is where the family relationship came from.

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Q: Dod Mary Queen of Scots kill Elizabeth's family member?
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