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Alfred I. du Pont amassed his wealth primarily through his family's chemical company, E.I. du Pont de Nemours and Company, which was founded by his ancestors in the early 19th century. He played a significant role in expanding the company's operations and diversifying its product lines, particularly in explosives and later in materials like nylon and other chemicals. Additionally, he invested in various other ventures, including banking and real estate, which further contributed to his fortune. His business acumen and strategic investments solidified his status as a prominent figure in American industry.

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