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Bioburden is typically counted by collecting a sample from the product or surface and culturing it on a suitable growth medium under controlled conditions. After an incubation period, colonies that form are counted, providing an estimate of the viable microbial load. Common techniques include plate counting, where dilutions of the sample are plated and colonies are counted, and methods like membrane filtration for larger volumes. It's essential to follow standardized protocols to ensure accurate and reproducible results.

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