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The LAL assay, or Limulus Amebocyte Lysate assay, is a test used to detect endotoxins, which are toxic substances released from the cell walls of gram-negative bacteria. It utilizes lysate derived from the blood cells of the horseshoe crab (Limulus polyphemus), which coagulates in the presence of endotoxins. When a sample is introduced to the lysate, the presence of endotoxins activates a cascade of reactions that leads to gel formation, indicating a positive result. This assay is widely used in pharmaceuticals and medical device industries to ensure product safety.

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What is the LAL assay?

The Limulus Amebocyte Lysate (LAL) assay is a test used to detect bacterial endotoxins in pharmaceutical and medical device products. LAL is derived from the blood cells of the horseshoe crab and can rapidly detect even very small amounts of endotoxins. It is a sensitive and widely used method in the pharmaceutical industry to ensure product quality and safety.


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