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Moisture can lead to the degradation of Mueller-Hinton II agar plates by promoting the growth of contaminants and altering the biochemical environment necessary for accurate antimicrobial susceptibility testing. Excess moisture can also cause the agar to become uneven or to lose its structural integrity, potentially affecting the diffusion of antibiotics. Additionally, high humidity can result in the formation of condensation, which may interfere with the interpretation of results by causing the zones of inhibition to be blurred or distorted.

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