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The response factor is determined by measuring the detector response to a known concentration of an analyte. This is typically done by preparing a series of standard solutions at known concentrations and measuring their responses using the analytical instrument. The response factor is then calculated by dividing the detector response (e.g., peak area or height) by the concentration of the analyte, establishing a relationship that can be used for quantifying unknown samples. It's essential to ensure that the measurements are within the linear range of the detector for accurate results.

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