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Joanne read that certain perfumes esters would agitate bees. Because perfumes formulas are secret, she decided to determine whether the unknown Ester X was present in four different perfumes by observing the bees behavior. She placed a saucer containing 10 ml of the first perfume 3 m from the hive. She recorded the time required for the bees to emerge and make observations on their behavior. After 30 minutes recovery period. She tested the second, third and fourth perfumes. All experiment were considered on the same day when the weather condition were similar that is air temperature and wind.

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