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Koch's Postulates (Procedures):

Step 1. ASSOCIATION: The suspected pathogen must be consistently associated with the diseased plant (or animal).

Step 2. ISOLATION: The pathogen must be isolated and grown in pure culture and its characteristics described.

Step 3. INOCULATION: The pathogen from pure culture is inoculated into a healthy plant of the same species or variety and it must produce the same symptoms and signs.

Step 4. RE-ISOLATION: The pathogen is re-isolated from the inoculated plant and its characteristics must be the same as the organism initially isolated in step 2.

Ask a question- Is this a pathogen

Do Research- find the suspected pathogen and isolate it and grow a pure culture

Construct a Hypothesis-

Test- Inject suspected pathogen into healthy organisms

Analyze and Conclude- If suspected pathogen has produced same signs and symptoms in multiple tests on same organisms then it is indeed a pathogen

Communicate Results

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12y ago
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12y ago

Possibly with his use of laboratory skills such as: use of sterile technique, comparing and contrasting

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