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Three methods are used mainly to cultivate viruses: 1ANIMAL INOCULATION: First used human volenteers for work on yellow virus fever. Land Steiner and Poper used monkey for isolation of polioviruses Most widely used animal is white mice. Animal inoculation have several disadvantages as Immunity may interfare with viral growth and the animal may harbour latent viruses. 2 EMBRYONATED EGGS: Method was developed by Burnet and virus can be inoculated in several routes as in chorio allantoic membrane, allontoic membrane, in to ammniotic cavity and also in yolk sac. 3 TISSUE CULTURE: The culture of whole organ or tissue fragment as well as dispersed cells on a nutreint medium is used. It can be done by three ways. A)ORGAN CULTURE: Embryonic organ or mall tissue fragments are cultured in vitro in such a manner that they retain their tissue architecture. B) EXPLANT CULTURE: Fragment of minced tissue can be grown on explant e.g. Adenoid tissue explant culture for adenovirus. C) CELL CULTURE: Dispersed or loosed cells are used in that method to grwo viruses . First washed with salt solution they dissociated in component cell by using protolytic enzyme such as trypsin and mechanical shaking. Presence of EDTA helps in disspersion of cells. It can be again divided in different parts. Primary culture Diploid culture and Contineous culture.
What else can I help you with?
Are bacteria larger than viruses?
no bacteria is larger than viruses
Infer how some viruses might can be helpful?
viruses can be helpful because, some viruses can be used for cures. for example, USA. researcher suggest that adeno-associated viruses type 2 can Promises to be a breakthrough in cancer therapy
What is the name of person who studies viruses?
a virologist studies viruses
How does sneezing and coughing protect the body from viruses?
They help to expel the viruses caught in the respiratory mucus.
Which is difference between bacteria and viruses that shows that bacteria are living organisms and viruses not?
Bacteria are able to reproduce on their own while viruses need a host to "hijack" so they can reproduce. Bacteria have metabolisms, catabolic and anabolic biochemical reactions going on in their cells all the time. Viruses do not. Bacteria can grow in size to a certain extent while viruses do not change in this regard. The definition of living things classify bacteria as a living thing, while viruses are not.
What are the different methods of culturing plant tissue?
BY ripping it apart
How can you determine the presence of bacteria in your environmental samples?
To determine the presence of bacteria in environmental samples, you can use techniques such as culturing, microscopy, and molecular methods like polymerase chain reaction (PCR) or sequencing. Culturing involves growing bacteria on specific media, while microscopy allows you to visualize bacteria under a microscope. Molecular methods can detect bacterial DNA in the sample, providing a more accurate and sensitive detection method.
What does diagnosis for leishmaniasis involve?
taking a scraping from a lesion, preparing it in a laboratory, and examining it under a microscope to demonstrate the causative protozoan. Other methods that have been used include culturing a sample
What is the hobby of culturing of bees?
That is apiculture
Why culturing of mitochondria not possible?
They are not individual organisms.
What is culturing in bees called?
Apiculture or beekeeping.
What arwe the most popular methods of getting rid of computer viruses?
Well their are many different kinds of viruses but most all go down by AVG- anti virus
What is culture fishery?
production of fish in given water bodies like ponds , lakes , reservoir etc. using scientific methods iike puding ,breeding etc so as to enhance the output. it is called culturing fishery............................
How do sanitation methods stop infectious diseases from spreading?
When you sanitize something, you are removing any germs or viruses that could be on the item. this completely stops the spread of germs and viruses, as long as that item remains sterile.
What are the two methods of viruses replication?
The only way viruses can replicate is by parasitizing living cells and using the cell's mechanisms to replicate their genetic materials and protein components.
What other technique produces identical plants?
tissue culturing
What are the past and present participle forms of the word culture?
The past participle is cultured. The present participle is culturing.
